Kastl

Versatile desktop behavioral arena, video recording, and analysis.

Kastl is a desktop sized behavioural arena for fish and other animals. The economic price and larger assay size allow diverse behavioural experiments with high throughput. Kastl is designed to be as versatile as possible to allow the application for several different model species and arenas. Among others, Kastl is currently used for larval zebrafish, several adult fish species, marine bristleworms and C.elegans.

The core of Kastl is a Motif recording unit, making it the most advanced, highest resolution and framerate recording solution currently available.

Kastl seamlessly integrates with Loopy for tracking and behavioral analysis. It provides a simple, remote controllable web-browser based interface for managing and analyzing your data, tracking and plotting. It supports both Loopy on-site and Loopy online, providing different processing options to support any budget.

A standard configuration of Kastl supports the following features

  • 24/7 operation
  • High throughput
  • Light for stimulation (white or color/RGB)
  • Individual light conditions and easily customized lighting programs
  • 940nm IR lighting
  • Several imaging areas available (from 100x100mm to 300x300mm as desktop version, up to 1.2m as standalone)
  • High frame rate and resolution

Kastl is available with the following additions

  • Temperature control
  • Custom lighting spectra
  • Light, temperature, or other environmental sensors
  • Multi-camera recording
  • 3D tracking
  • Data analysis and backup online with loopb.io

Application examples

High throughput screening of multi well plates

Making use of latest higher resolution cameras, Kastl can record not only a single multi-well plate but several at the same time, increasing your throughput.

Desktop size schooling analysis

Kastl can be used with a custom circular schooling arena for investigating group our schooling behavior in a small form factor assay.

High throughput circadian analysis with non model organisms

Kastl is not restricted to use with fish. See here for a case study showing how Kastl, with custom lighting control, is used for investigating circadian and circalunar mechanisms in marine bristelworms.

High speed recording

Kastl can be equipped with an ultra bright IR LED background illumination and a high speed camera delivering 700 frames per second at VGA resolution constantly (24/7). This is used to investigate kinematic movements with temporal and spatial resolution that are not available elsewhere.

Tracking Output

Besides standard activity measurement like speed and distance moved, more advanced measures are possible. For example, frequency and inter-individual distance for zebrafish, or bending angle for worms of any kind. The following measurements are automatically calculated;

  • Distance moved and velocity
  • Spatial position (heatmaps)
  • Curvature (skeleton fitting)
  • Inter-individual distance (schooling)
  • Size of individuals